Synonym | Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB |
Species | Bovine |
Protein Accession | P03969 |
Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) |
Endotoxin Level | ≤100 EU/mg by the LAL method |
Biological Activity | Measured in a cell proliferation assay using NIH3T3 mouse embryonicfibroblast cells. The ED50 for this effect is ≤2.0 ng/mL |
Expression System | E.coli |
Fusion Tag | His Tag |
Predicted Molecular Mass | 17.4 kDa |
Formulation | Lyophilized from a 0.22 μm-filtered solution containing 10mM Tris,200mM Nacl, 5% mannitol, pH 8.0. |
Reconstitution | It is recommended to redissolve in sterile deionized water. |
Storage & Stability | 36 months at -20°C to -80°C in lyophilized state 6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |